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immunofluorescence microscope  (Olympus)


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    Structured Review

    Olympus immunofluorescence microscope
    Immunofluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 6126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+microscope/IX73+Inverted+Microscope+Currently+integrated+into+IXplore+Systems/pmc13678848-72-6-9
    Average 97 stars, based on 6126 article reviews
    immunofluorescence microscope - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Evaluation of Hep-2 Cell Patterns: How Often Do We Report Cytoplasmic and Mitotic Patterns?
    Article Snippet: During the study period, samples were tested at 1/100 dilution using HEp-20-10/Liver (Monkey) Mosaic IIF kit (Euroimmun, Germany) in accordance with the manufacturer's recommendations. .. The tests were visually evaluated using an immunofluorescence microscope (Olympus BX53) after staining procedures performed according to standard protocols. ..

    Article Title: Evolutionary Dynamics and Transboundary Spread of Senecavirus A: The Role of Recombination and Global Swine Trade
    Article Snippet: The cells were counterstained with 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI; Sigma–Aldrich, D8417). .. Fluorescence signals were examined using an immunofluorescence microscope (IX73; Olympus, Tokyo, Japan). .. Viral RNA was extracted from clarified supernatants of SVA‐infected BHK‐21 cells using the TIANGEN Viral RNA Kit, and cDNA was synthesized with random primers using the RevertAid First Strand cDNA Synthesis Kit.

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. Finally, an immunofluorescence microscope (Olympus BX53, Japan) was used for observation. .. Cell Culture RAW264.7 Culture RAW264.7 cells were cultured in 10% fetal bovine serum (FBS), 100 U/mL penicillin, 0.1 mg/mL streptomycin and 0.25 μg/mL amphotericin B in DMEM at 37 °C in a 5% CO2 thermostatic cell culture incubator (Thermo Scientific Forma Steri-Cycle i160).

    Article Title: Vincristine enhances doxorubicin cardiotoxicity in human iPSC-derived cardiomyocytes: Partial attenuation by SGLT2 inhibition.
    Article Snippet: Background: Doxorubicin is a well-known cardiotoxic chemotherapeutic agent.. Whether its combination with vincristine exacerbates cardiotoxicity and the underlying mechanisms remains unclear.. Methods: Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) were treated with doxorubicin, vincristine, or both.

    Article Title: A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange
    Article Snippet: .. After thorough washing, the sections were incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rat IgG (diluted 1:200 in PBS; Abclonal, Wuhan, China), counterstained with 4′, 6-diamidino-2-phenylindole (DAPI), and visualized using an immunofluorescence microscope (BX53, Olympus, Japan). ..

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. Finally, an immunofluorescence microscope (Olympus BX53, Japan) was used for observation. .. RAW264.7 cells were cultured in 10% fetal bovine serum (FBS), 100 U/mL penicillin, 0.1 mg/mL streptomycin and 0.25 μg/mL amphotericin B in DMEM at 37 °C in a 5% CO 2 thermostatic cell culture incubator (Thermo Scientific Forma Steri-Cycle i160).

    Article Title: The Role of Placental MFF-Mediated Mitochondrial Fission in Gestational Diabetes Mellitus
    Article Snippet: .. Placental samples were subjected to immunostaining with an antibody targeting MFF (1:200), followed by visualization using an immunofluorescence microscope (Olympus BX53, Japan). ..

    Article Title: A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange.
    Article Snippet: .. After thorough washing, the sections were incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rat IgG (diluted 1:200 in PBS; Abclonal, Wuhan, China), counterstained with 4′, 6-diamidino-2-phenylindole (DAPI), and visualized using an immunofluorescence microscope (BX53, Olympus, Japan). ..

    Microscopy:

    Article Title: Evaluation of Hep-2 Cell Patterns: How Often Do We Report Cytoplasmic and Mitotic Patterns?
    Article Snippet: During the study period, samples were tested at 1/100 dilution using HEp-20-10/Liver (Monkey) Mosaic IIF kit (Euroimmun, Germany) in accordance with the manufacturer's recommendations. .. The tests were visually evaluated using an immunofluorescence microscope (Olympus BX53) after staining procedures performed according to standard protocols. ..

    Article Title: Evolutionary Dynamics and Transboundary Spread of Senecavirus A: The Role of Recombination and Global Swine Trade
    Article Snippet: The cells were counterstained with 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI; Sigma–Aldrich, D8417). .. Fluorescence signals were examined using an immunofluorescence microscope (IX73; Olympus, Tokyo, Japan). .. Viral RNA was extracted from clarified supernatants of SVA‐infected BHK‐21 cells using the TIANGEN Viral RNA Kit, and cDNA was synthesized with random primers using the RevertAid First Strand cDNA Synthesis Kit.

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. Finally, an immunofluorescence microscope (Olympus BX53, Japan) was used for observation. .. Cell Culture RAW264.7 Culture RAW264.7 cells were cultured in 10% fetal bovine serum (FBS), 100 U/mL penicillin, 0.1 mg/mL streptomycin and 0.25 μg/mL amphotericin B in DMEM at 37 °C in a 5% CO2 thermostatic cell culture incubator (Thermo Scientific Forma Steri-Cycle i160).

    Article Title: Vincristine enhances doxorubicin cardiotoxicity in human iPSC-derived cardiomyocytes: Partial attenuation by SGLT2 inhibition.
    Article Snippet: Background: Doxorubicin is a well-known cardiotoxic chemotherapeutic agent.. Whether its combination with vincristine exacerbates cardiotoxicity and the underlying mechanisms remains unclear.. Methods: Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) were treated with doxorubicin, vincristine, or both.

    Article Title: A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange
    Article Snippet: .. After thorough washing, the sections were incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rat IgG (diluted 1:200 in PBS; Abclonal, Wuhan, China), counterstained with 4′, 6-diamidino-2-phenylindole (DAPI), and visualized using an immunofluorescence microscope (BX53, Olympus, Japan). ..

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. Finally, an immunofluorescence microscope (Olympus BX53, Japan) was used for observation. .. RAW264.7 cells were cultured in 10% fetal bovine serum (FBS), 100 U/mL penicillin, 0.1 mg/mL streptomycin and 0.25 μg/mL amphotericin B in DMEM at 37 °C in a 5% CO 2 thermostatic cell culture incubator (Thermo Scientific Forma Steri-Cycle i160).

    Article Title: The Role of Placental MFF-Mediated Mitochondrial Fission in Gestational Diabetes Mellitus
    Article Snippet: .. Placental samples were subjected to immunostaining with an antibody targeting MFF (1:200), followed by visualization using an immunofluorescence microscope (Olympus BX53, Japan). ..

    Article Title: A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange.
    Article Snippet: .. After thorough washing, the sections were incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rat IgG (diluted 1:200 in PBS; Abclonal, Wuhan, China), counterstained with 4′, 6-diamidino-2-phenylindole (DAPI), and visualized using an immunofluorescence microscope (BX53, Olympus, Japan). ..

    Staining:

    Article Title: Evaluation of Hep-2 Cell Patterns: How Often Do We Report Cytoplasmic and Mitotic Patterns?
    Article Snippet: During the study period, samples were tested at 1/100 dilution using HEp-20-10/Liver (Monkey) Mosaic IIF kit (Euroimmun, Germany) in accordance with the manufacturer's recommendations. .. The tests were visually evaluated using an immunofluorescence microscope (Olympus BX53) after staining procedures performed according to standard protocols. ..

    Fluorescence:

    Article Title: Evolutionary Dynamics and Transboundary Spread of Senecavirus A: The Role of Recombination and Global Swine Trade
    Article Snippet: The cells were counterstained with 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI; Sigma–Aldrich, D8417). .. Fluorescence signals were examined using an immunofluorescence microscope (IX73; Olympus, Tokyo, Japan). .. Viral RNA was extracted from clarified supernatants of SVA‐infected BHK‐21 cells using the TIANGEN Viral RNA Kit, and cDNA was synthesized with random primers using the RevertAid First Strand cDNA Synthesis Kit.

    Article Title: Vincristine enhances doxorubicin cardiotoxicity in human iPSC-derived cardiomyocytes: Partial attenuation by SGLT2 inhibition.
    Article Snippet: Background: Doxorubicin is a well-known cardiotoxic chemotherapeutic agent.. Whether its combination with vincristine exacerbates cardiotoxicity and the underlying mechanisms remains unclear.. Methods: Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) were treated with doxorubicin, vincristine, or both.

    Incubation:

    Article Title: A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange
    Article Snippet: .. After thorough washing, the sections were incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rat IgG (diluted 1:200 in PBS; Abclonal, Wuhan, China), counterstained with 4′, 6-diamidino-2-phenylindole (DAPI), and visualized using an immunofluorescence microscope (BX53, Olympus, Japan). ..

    Article Title: A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange.
    Article Snippet: .. After thorough washing, the sections were incubated with fluorescein isothiocyanate (FITC)-labeled goat anti-rat IgG (diluted 1:200 in PBS; Abclonal, Wuhan, China), counterstained with 4′, 6-diamidino-2-phenylindole (DAPI), and visualized using an immunofluorescence microscope (BX53, Olympus, Japan). ..

    Immunostaining:

    Article Title: The Role of Placental MFF-Mediated Mitochondrial Fission in Gestational Diabetes Mellitus
    Article Snippet: .. Placental samples were subjected to immunostaining with an antibody targeting MFF (1:200), followed by visualization using an immunofluorescence microscope (Olympus BX53, Japan). ..



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    Olympus immunofluorescence imaging
    IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and <t>immunofluorescence</t> staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.
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    Image Search Results


    IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and immunofluorescence staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.

    Journal: Journal of Orthopaedic Translation

    Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

    doi: 10.1016/j.jot.2025.101035

    Figure Lengend Snippet: IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and immunofluorescence staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.

    Article Snippet: The slices were sealed and stored at −20 °C for immunofluorescence imaging (FV 3000, Olympus, Japan).

    Techniques: Staining, Immunofluorescence, Fluorescence, Expressing, Western Blot, Control

    IChondrocyte-sEVs inhibited fibrosis and calcification in MSC-derived chondrocyte-pellets, as well as ameliorated impairment in OA chondrocytes. a Representative immunofluorescence images of MSC-derived chondrocytes pellets (n = 4). Scale bar: 20 μm b Quantitative analysis of mean fluorescence intensity for COLX and COLI across experimental groups (n = 4). c Microstructural characterization of MSC-derived chondrocyte pellets with μCT and SEM (n = 4). Calcified regions are indicated by green fluorescence, with calcium salt deposition marked by red arrows. Scale bar: white = 500 μm, black = 20 μm d Quantitative assessment of bone volume and bone volume percentage in MSC-derived chondrocytes pellets (n = 4). e OA chondrocytes proliferation was assessed over a 4-day period (n = 3). f Representative images illustrating cellular morphology, Safranin O staining, and Alcian blue staining of chondrocytes (n = 3). Scale bar:200 um. g Relative mRNA expression levels of COL2A1 and ACAN genes in chondrocytes (n = 6). h Western blotting analysis of COLII and ACAN in chondrocytes (n = 4). Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ### P < 0.001 vs. control group.

    Journal: Journal of Orthopaedic Translation

    Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

    doi: 10.1016/j.jot.2025.101035

    Figure Lengend Snippet: IChondrocyte-sEVs inhibited fibrosis and calcification in MSC-derived chondrocyte-pellets, as well as ameliorated impairment in OA chondrocytes. a Representative immunofluorescence images of MSC-derived chondrocytes pellets (n = 4). Scale bar: 20 μm b Quantitative analysis of mean fluorescence intensity for COLX and COLI across experimental groups (n = 4). c Microstructural characterization of MSC-derived chondrocyte pellets with μCT and SEM (n = 4). Calcified regions are indicated by green fluorescence, with calcium salt deposition marked by red arrows. Scale bar: white = 500 μm, black = 20 μm d Quantitative assessment of bone volume and bone volume percentage in MSC-derived chondrocytes pellets (n = 4). e OA chondrocytes proliferation was assessed over a 4-day period (n = 3). f Representative images illustrating cellular morphology, Safranin O staining, and Alcian blue staining of chondrocytes (n = 3). Scale bar:200 um. g Relative mRNA expression levels of COL2A1 and ACAN genes in chondrocytes (n = 6). h Western blotting analysis of COLII and ACAN in chondrocytes (n = 4). Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ### P < 0.001 vs. control group.

    Article Snippet: The slices were sealed and stored at −20 °C for immunofluorescence imaging (FV 3000, Olympus, Japan).

    Techniques: Derivative Assay, Immunofluorescence, Fluorescence, Staining, Expressing, Western Blot, Control